Journal:

Article Title: Comparison of a New Quantitative ompA -Based Real-Time PCR TaqMan Assay for Detection of Chlamydia pneumoniae DNA in Respiratory Specimens with Four Conventional PCR Assays

doi: 10.1128/JCM.41.2.592-600.2003

Figure Lengend Snippet: Microorganisms a used to test the specificity and cross-reactivity of the real-time PCR assay designed to detect the ompA gene of C. pneumoniae

Article Snippet: To test for the specificity and cross-reactivity of the real-time PCR assay, 37 microorganisms from the American Type Culture Collection, the Culture Collection of the University of Goeteborg (CCUG), and the Quality Assurance Laboratory of the Central Public Health Laboratory at Colindale (London, United Kingdom) were tested and are listed in Table . table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Species Source (strain or reference) C. pneumoniae TW-183; ATCC (VR-2282) C. pneumoniae AR-39; ATCC (53592) C. pneumoniae MUL-1; M. Maass C. pneumoniae WIEN I ( 3 ) C. pneumoniae WIEN II ( 3 ) C. pneumoniae WIEN III ( 3 ) C. pneumoniae Local respiratory isolate C. trachomatis ATCC (VR-902B) Staphylococcus aureus ATCC (25923) Staphylococcus lugdunensis ATCC (700328) Staphylococcus epidermidis QC b Streptococcus pneumoniae ATCC (6303) Streptococcus pyogenes ATCC (19615) Streptococcus mitis QC Streptococcus sanguis QC Streptococcus uberis QC Streptococcus milleri QC Enterococcus faecalis QC Haemophilus influenzae ATCC (10211) Haemophilus parainfluenzae QC Escherichia coli ATCC (11229) Mycoplasma pneumoniae CCUG (10119) Neisseria perflava CCUG (17915) Neisseria subflava CCUG (23930) Corynebacterium diphtheriae QC Bordetella pertussis QC Moraxella catarrhalis QC Legionella pneumophila QC Klebsiella pneumoniae QC Eikenella corrodens QC Pseudomonas aeruginosa QC Stenotrophomonas maltophilia QC Bacteroides fragilis QC Peptostreptococcus anaerobius QC Lactobacillus casei QC Prevotella corporis QC Porphyromonas asaccharolyticus QC Open in a separate window a A total of 37 isolates were tested. b QC, quality control strains; the isolates were obtained from the Quality Assurance Laboratory of the Central Public Health Laboratory at Colindale, London, United Kingdom, for the purpose of quality control.

Techniques: Real-time Polymerase Chain Reaction

Journal: Clinical Cases in Mineral and Bone Metabolism

Article Title: Fracture healing and drug therapies in osteoporosis

doi:

Figure Lengend Snippet: List of articles included in our systematic review on fracture healing and drug therapies and its effect on callus formation.

Article Snippet: Wang JW., et al. 2007 , Animal study , 130 2-month-old female Sprague- Dawley rats with initial body weight of 178-201 g. All the rats were randomly subjected to either OVX (n=120) or sham surgery (n=10). , All the OVX rats were used to establish open fracture models and fixed with intermedullary nails. Transverse open fracture model was made in the proximal one-third of the right tibia. , Simvastatin or vehicle drugs were injected subcutaneously to the fracture site once on the day of fracture and twice in 5 days thereafter. A solution of 5% simvastatin (Hisun Pharmaceutical Co. Ltd. China) simvastatin was diffused in PBS with 2 dimethylsulfoxide and 0.1% BSA. Each injection contained 5 mg/kg simvastatin of the SVS group and an equal volume (about 30 μl-50 μl) of vehicle solution to the vehicle groups. , Substantial histological differences between the OVX + vehicle and OVX + SVS callus zones were visible at 1 and 2 weeks after treatment. The callus cross-section area in simvastatin-treated rats was significantly enlarged by 21.3% at 1 week (20.22±3.42 mm 2 vs. 16.67±4.02 mm 2 ) and by 21.5% at 2 weeks (23.63±3.25 mm 2 vs. 19.45±3.19 mm 2 ) than the vehicle group , Increased.

Techniques: Injection, Standard Deviation, Amplification, Reverse Transcription Polymerase Chain Reaction, Expressing